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african green monkey kidney fibroblast derived cos 7 cells  (ATCC)


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    ATCC african green monkey kidney fibroblast derived cos 7 cells
    (a) Schematic showing the experimental plan for CgA/PA interaction monitoring in living cells by the FLIM-FRET technique. Following the overexpression of CgA- or CgAΔPABD-mKate2 <t>in</t> <t>COS-7</t> cells, their secretion was induced during 5 min using a BaCl 2 solution. Then cells were washed and incubated 5 min with PA-ATTO647N. (b) Principle of FLIM-FRET technique to track the lifetime of the fluorescence donor (mKate2) without or with CgA/PA-ATTO647N interaction. The more CgA-mKate2 interacts with PA, the more the lifetime of mKate2 decreases due to its energy transfer to the acceptor fluorophore (ATTO647N). (c) Confocal observations of COS-7 cells overexpressing CgA-mKate2 or CgAΔPABD-mKate2 (green) after secretion stimulation and incubation with the PA-ATTO647N probe (magenta). Scale bars: 10 µm. The regions delimited by a white square on left images are enlarged to show the distribution of each fluorescent molecule. Scale bars: 2 µm. (d) Confocal observations of COS-7 cells overexpressing CgA-mKate2 or CgAΔPABD-mKate2, after cell incubation with PA-ATTO647N, showing the intensity of mKate2 fluorescence (grey), and mKate2 lifetime between 3 and 1.8 ns (rainbow color bar). The regions delimited by a white square are enlarged to show mKate2 lifetime at the plasma membrane. Scale bars: 5 µm. (e) Representative phasor plots showing the CgA-mKate2 lifetime at the level of the whole image and at the plasma membrane zone. Green line corresponds to 2.4 ns and yellow line to 2 ns, used as visual landmarks. (f) Representative phasor plot showing the CgAΔPABD-mKate2 lifetime at the level of the whole image. Green line corresponds to 2.4 ns and yellow line to 2 ns, used as visual landmarks. (g) Quantification of CgA-mKate2 or CgAΔPABD-mKate2 lifetime at the level of the plasma membrane (PM) or secretory granule (SG) without (-) or with (+) PA-ATTO647N. nd: not detected. Kruskall-Wallis test * p<0,05, each point representing the mean mKate2 lifetime in a ROI at the PM or SG level of 2 to 3 cells.
    African Green Monkey Kidney Fibroblast Derived Cos 7 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 7949 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/african+green+monkey+kidney+derived+cos+7+cells/COS-7/bio_rxiv__64898__2026__01__29__699889-141-0-7
    Average 99 stars, based on 7949 article reviews
    african green monkey kidney fibroblast derived cos 7 cells - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Chromogranin A regulates the dynamics of neurosecretion through its interaction with phosphatidic acid"

    Article Title: Chromogranin A regulates the dynamics of neurosecretion through its interaction with phosphatidic acid

    Journal: bioRxiv

    doi: 10.64898/2026.01.29.699889

    (a) Schematic showing the experimental plan for CgA/PA interaction monitoring in living cells by the FLIM-FRET technique. Following the overexpression of CgA- or CgAΔPABD-mKate2 in COS-7 cells, their secretion was induced during 5 min using a BaCl 2 solution. Then cells were washed and incubated 5 min with PA-ATTO647N. (b) Principle of FLIM-FRET technique to track the lifetime of the fluorescence donor (mKate2) without or with CgA/PA-ATTO647N interaction. The more CgA-mKate2 interacts with PA, the more the lifetime of mKate2 decreases due to its energy transfer to the acceptor fluorophore (ATTO647N). (c) Confocal observations of COS-7 cells overexpressing CgA-mKate2 or CgAΔPABD-mKate2 (green) after secretion stimulation and incubation with the PA-ATTO647N probe (magenta). Scale bars: 10 µm. The regions delimited by a white square on left images are enlarged to show the distribution of each fluorescent molecule. Scale bars: 2 µm. (d) Confocal observations of COS-7 cells overexpressing CgA-mKate2 or CgAΔPABD-mKate2, after cell incubation with PA-ATTO647N, showing the intensity of mKate2 fluorescence (grey), and mKate2 lifetime between 3 and 1.8 ns (rainbow color bar). The regions delimited by a white square are enlarged to show mKate2 lifetime at the plasma membrane. Scale bars: 5 µm. (e) Representative phasor plots showing the CgA-mKate2 lifetime at the level of the whole image and at the plasma membrane zone. Green line corresponds to 2.4 ns and yellow line to 2 ns, used as visual landmarks. (f) Representative phasor plot showing the CgAΔPABD-mKate2 lifetime at the level of the whole image. Green line corresponds to 2.4 ns and yellow line to 2 ns, used as visual landmarks. (g) Quantification of CgA-mKate2 or CgAΔPABD-mKate2 lifetime at the level of the plasma membrane (PM) or secretory granule (SG) without (-) or with (+) PA-ATTO647N. nd: not detected. Kruskall-Wallis test * p<0,05, each point representing the mean mKate2 lifetime in a ROI at the PM or SG level of 2 to 3 cells.
    Figure Legend Snippet: (a) Schematic showing the experimental plan for CgA/PA interaction monitoring in living cells by the FLIM-FRET technique. Following the overexpression of CgA- or CgAΔPABD-mKate2 in COS-7 cells, their secretion was induced during 5 min using a BaCl 2 solution. Then cells were washed and incubated 5 min with PA-ATTO647N. (b) Principle of FLIM-FRET technique to track the lifetime of the fluorescence donor (mKate2) without or with CgA/PA-ATTO647N interaction. The more CgA-mKate2 interacts with PA, the more the lifetime of mKate2 decreases due to its energy transfer to the acceptor fluorophore (ATTO647N). (c) Confocal observations of COS-7 cells overexpressing CgA-mKate2 or CgAΔPABD-mKate2 (green) after secretion stimulation and incubation with the PA-ATTO647N probe (magenta). Scale bars: 10 µm. The regions delimited by a white square on left images are enlarged to show the distribution of each fluorescent molecule. Scale bars: 2 µm. (d) Confocal observations of COS-7 cells overexpressing CgA-mKate2 or CgAΔPABD-mKate2, after cell incubation with PA-ATTO647N, showing the intensity of mKate2 fluorescence (grey), and mKate2 lifetime between 3 and 1.8 ns (rainbow color bar). The regions delimited by a white square are enlarged to show mKate2 lifetime at the plasma membrane. Scale bars: 5 µm. (e) Representative phasor plots showing the CgA-mKate2 lifetime at the level of the whole image and at the plasma membrane zone. Green line corresponds to 2.4 ns and yellow line to 2 ns, used as visual landmarks. (f) Representative phasor plot showing the CgAΔPABD-mKate2 lifetime at the level of the whole image. Green line corresponds to 2.4 ns and yellow line to 2 ns, used as visual landmarks. (g) Quantification of CgA-mKate2 or CgAΔPABD-mKate2 lifetime at the level of the plasma membrane (PM) or secretory granule (SG) without (-) or with (+) PA-ATTO647N. nd: not detected. Kruskall-Wallis test * p<0,05, each point representing the mean mKate2 lifetime in a ROI at the PM or SG level of 2 to 3 cells.

    Techniques Used: Over Expression, Incubation, Fluorescence, Clinical Proteomics, Membrane

    (a) TIRF-M imaging of COS-7 cells overexpressing and secreting CgA- and CgAΔPABD-pHluorin after 2 mM BaCl 2 stimulation. SG leading to exocytic events on whole cells are surrounded and indicated by yellow arrows. Scale bars: 10 µm. Below are represented typical images of CgA- or CgAΔPABD-pHluorin exocytosis events. (b) Quantification of the number of exocytosis events in COS-7 cells overexpressing CgA-pHluorin and CgAΔPABD-pHluorin after their stimulation. (n=4 independent experiments; 8 cells in CgA condition and 7 cells in CgAΔPABD condition). Data are represented as mean ± SEM. Each point is one analyzed cell and each color is an independent experiment. **p < 0.01, Mann-Whitney test. (c) Typical images of CgA-EGFP or CgAΔPABD-EGFP exocytosis events. Scale bar: 500 nm. (d) Curves represent the normalized variation of the fluorescence intensity of single exocytic events during 10 s from CgA-or CgAΔPABD-EGFP overexpressing COS-7 cells after 2 mM BaCl 2 stimulation. The mean ± SEM of 75 exocytosis of 5 different CgA-EGFP overexpressing cells in 3 independent experiments and the mean of 65 exocytic events of 7 different CgAΔPABD-EGFP overexpressing cells in 3 independent experiments. Data have been normalized between 0 (before exocytosis) and 1 (maximum intensity). (e) Plot representing the mean ± SEM of area under curves from CgA-EGFP or CgAΔPABD-EGFP secretion kinetic. Each point represents one exocytosis event, and one color represents one analyzed cell. ****p < 0.0001, Mann-Whitney test.
    Figure Legend Snippet: (a) TIRF-M imaging of COS-7 cells overexpressing and secreting CgA- and CgAΔPABD-pHluorin after 2 mM BaCl 2 stimulation. SG leading to exocytic events on whole cells are surrounded and indicated by yellow arrows. Scale bars: 10 µm. Below are represented typical images of CgA- or CgAΔPABD-pHluorin exocytosis events. (b) Quantification of the number of exocytosis events in COS-7 cells overexpressing CgA-pHluorin and CgAΔPABD-pHluorin after their stimulation. (n=4 independent experiments; 8 cells in CgA condition and 7 cells in CgAΔPABD condition). Data are represented as mean ± SEM. Each point is one analyzed cell and each color is an independent experiment. **p < 0.01, Mann-Whitney test. (c) Typical images of CgA-EGFP or CgAΔPABD-EGFP exocytosis events. Scale bar: 500 nm. (d) Curves represent the normalized variation of the fluorescence intensity of single exocytic events during 10 s from CgA-or CgAΔPABD-EGFP overexpressing COS-7 cells after 2 mM BaCl 2 stimulation. The mean ± SEM of 75 exocytosis of 5 different CgA-EGFP overexpressing cells in 3 independent experiments and the mean of 65 exocytic events of 7 different CgAΔPABD-EGFP overexpressing cells in 3 independent experiments. Data have been normalized between 0 (before exocytosis) and 1 (maximum intensity). (e) Plot representing the mean ± SEM of area under curves from CgA-EGFP or CgAΔPABD-EGFP secretion kinetic. Each point represents one exocytosis event, and one color represents one analyzed cell. ****p < 0.0001, Mann-Whitney test.

    Techniques Used: Imaging, MANN-WHITNEY, Fluorescence

    Related Articles

    Immunostaining:

    Article Title: I Ks response to protein kinase A-dependent KCNQ1 phosphorylation requires direct interaction with microtubules
    Article Snippet: African green monkey kidney-derived COS-7 cells (ATCC, Manassas, VA), guinea pig whole heart, rabbit anti-β-tubulin polyclonal antibody (Santa Cruz Biotechnology), goat anti-KCNQ1-C-terminus (Ct-KCNQ1) polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-KCNQ1 polyclonal antibody (Alomone Labs, Jerusalem, Israel), rabbit anti-Yotiao antibody (Zimed Laboratory Inc. or Fusion Antibodies), rabbit anti-phospho-S27 KCNQ1 antibody, 17 rabbit anti-phospho- specific Yotiao antibody, 14 mouse anti-vesicular stomatitis virus (VSV) antibody (Sigma–Aldrich, St Louis, MO), and secondary horseradish peroxidase-coupled antibody (Santa Cruz Biotechnology) were used. .. Immunostaining was performed on COS-7 cells and on freshly isolated cardiomyocytes from guinea pigs using rabbit anti-β-tubulin, mouse anti-β-tubulin, goat anti-Ct-KCNQ1 polyclonal, or rabbit anti-KCNQ1 antibodies and Alexa A488-conjugated anti-rabbit or Alexa A568-conjugated anti-goat secondary antibodies (Molecular Probes). .. Immunostaining was performed on COS-7 cells and on freshly isolated cardiomyocytes from guinea pigs using rabbit anti-β-tubulin, mouse anti-β-tubulin, goat anti-Ct-KCNQ1 polyclonal, or rabbit anti-KCNQ1 antibodies and Alexa A488-conjugated anti-rabbit or Alexa A568-conjugated anti-goat secondary antibodies (Molecular Probes).

    Isolation:

    Article Title: I Ks response to protein kinase A-dependent KCNQ1 phosphorylation requires direct interaction with microtubules
    Article Snippet: African green monkey kidney-derived COS-7 cells (ATCC, Manassas, VA), guinea pig whole heart, rabbit anti-β-tubulin polyclonal antibody (Santa Cruz Biotechnology), goat anti-KCNQ1-C-terminus (Ct-KCNQ1) polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-KCNQ1 polyclonal antibody (Alomone Labs, Jerusalem, Israel), rabbit anti-Yotiao antibody (Zimed Laboratory Inc. or Fusion Antibodies), rabbit anti-phospho-S27 KCNQ1 antibody, 17 rabbit anti-phospho- specific Yotiao antibody, 14 mouse anti-vesicular stomatitis virus (VSV) antibody (Sigma–Aldrich, St Louis, MO), and secondary horseradish peroxidase-coupled antibody (Santa Cruz Biotechnology) were used. .. Immunostaining was performed on COS-7 cells and on freshly isolated cardiomyocytes from guinea pigs using rabbit anti-β-tubulin, mouse anti-β-tubulin, goat anti-Ct-KCNQ1 polyclonal, or rabbit anti-KCNQ1 antibodies and Alexa A488-conjugated anti-rabbit or Alexa A568-conjugated anti-goat secondary antibodies (Molecular Probes). .. Immunostaining was performed on COS-7 cells and on freshly isolated cardiomyocytes from guinea pigs using rabbit anti-β-tubulin, mouse anti-β-tubulin, goat anti-Ct-KCNQ1 polyclonal, or rabbit anti-KCNQ1 antibodies and Alexa A488-conjugated anti-rabbit or Alexa A568-conjugated anti-goat secondary antibodies (Molecular Probes).

    Cell Culture:

    Article Title: Production of bioactive gastrin from the non-endocrine cell lines CHO and COS-7.
    Article Snippet: .. Cell culture and DNA transfection by electroporation A Ham’s F12 medium, supplemented with 5% fetal bovine serum (FBS) (Gibco, Grand Island, NY), was used to culture the Chinese hamster ovary-derived CHO-K2 cells (ATCC CCL 61), and a Dulbccco’s Modified Eagle’s medium (Sigma, St Louis, MO) with 10% FBS was used to culture the African green monkey kidney-derived COS-7 cells (ATCC CRL 1651). ..

    Transfection:

    Article Title: Production of bioactive gastrin from the non-endocrine cell lines CHO and COS-7.
    Article Snippet: .. Cell culture and DNA transfection by electroporation A Ham’s F12 medium, supplemented with 5% fetal bovine serum (FBS) (Gibco, Grand Island, NY), was used to culture the Chinese hamster ovary-derived CHO-K2 cells (ATCC CCL 61), and a Dulbccco’s Modified Eagle’s medium (Sigma, St Louis, MO) with 10% FBS was used to culture the African green monkey kidney-derived COS-7 cells (ATCC CRL 1651). ..

    Article Title: Mutation in ST6GALNAC5 identified in family with coronary artery disease
    Article Snippet: .. The vectors were transfected into African green monkey kidney derived COS-7 cells (ATCC, Rockville, MD, USA) and ST6GALNAC5 expression in transfected cells was shown by RT-PCR and by Western blotting. .. In the Western blot experiments, monoclonal mouse M2-anti-FLAG (Sigma-Aldrich, Munich, Germany), anti-human sialyltransferase 7e rabbit (abcam, Cambridge, MA, USA), or anti-human lamin B goat (Santa Cruz Biotechnology, Santa Cruz, CA, USA) primary antibody and appropriate secondary anti-IgG antibody coupled to horseradish peroxidase were used.

    Electroporation:

    Article Title: Production of bioactive gastrin from the non-endocrine cell lines CHO and COS-7.
    Article Snippet: .. Cell culture and DNA transfection by electroporation A Ham’s F12 medium, supplemented with 5% fetal bovine serum (FBS) (Gibco, Grand Island, NY), was used to culture the Chinese hamster ovary-derived CHO-K2 cells (ATCC CCL 61), and a Dulbccco’s Modified Eagle’s medium (Sigma, St Louis, MO) with 10% FBS was used to culture the African green monkey kidney-derived COS-7 cells (ATCC CRL 1651). ..

    Modification:

    Article Title: Production of bioactive gastrin from the non-endocrine cell lines CHO and COS-7.
    Article Snippet: .. Cell culture and DNA transfection by electroporation A Ham’s F12 medium, supplemented with 5% fetal bovine serum (FBS) (Gibco, Grand Island, NY), was used to culture the Chinese hamster ovary-derived CHO-K2 cells (ATCC CCL 61), and a Dulbccco’s Modified Eagle’s medium (Sigma, St Louis, MO) with 10% FBS was used to culture the African green monkey kidney-derived COS-7 cells (ATCC CRL 1651). ..

    Derivative Assay:

    Article Title: Mutation in ST6GALNAC5 identified in family with coronary artery disease
    Article Snippet: .. The vectors were transfected into African green monkey kidney derived COS-7 cells (ATCC, Rockville, MD, USA) and ST6GALNAC5 expression in transfected cells was shown by RT-PCR and by Western blotting. .. In the Western blot experiments, monoclonal mouse M2-anti-FLAG (Sigma-Aldrich, Munich, Germany), anti-human sialyltransferase 7e rabbit (abcam, Cambridge, MA, USA), or anti-human lamin B goat (Santa Cruz Biotechnology, Santa Cruz, CA, USA) primary antibody and appropriate secondary anti-IgG antibody coupled to horseradish peroxidase were used.

    Article Title: 14-3-3 Is a Regulator of the Cardiac Voltage-Gated Sodium Channel Nav1.5
    Article Snippet: .. Co-immunoprecipitation, pulldown and Western blotting The African green monkey kidney derived COS-7 cells (obtained from ATCC), were transiently co-transfected with pCI Nav1.5-GFP and pCI 14-3-3η expression vectors using BGTC-DOPE (7). .. For each experimental condition, confluent cells from two 30 mm dishes were harvested after 2 days and incubated in 500 μL ice-cold lysis buffer (in mmol/L: 150 NaCl, 1% IGEPAL, 50 tris-HCl, pH 8.0; all from Sigma) with 1 minitab of protease inhibitors (Roche) and 1 mmol/L PMSF (phenylmethylsulfonyl fluoride, Sigma) for 1 h at 4°C.

    Expressing:

    Article Title: Mutation in ST6GALNAC5 identified in family with coronary artery disease
    Article Snippet: .. The vectors were transfected into African green monkey kidney derived COS-7 cells (ATCC, Rockville, MD, USA) and ST6GALNAC5 expression in transfected cells was shown by RT-PCR and by Western blotting. .. In the Western blot experiments, monoclonal mouse M2-anti-FLAG (Sigma-Aldrich, Munich, Germany), anti-human sialyltransferase 7e rabbit (abcam, Cambridge, MA, USA), or anti-human lamin B goat (Santa Cruz Biotechnology, Santa Cruz, CA, USA) primary antibody and appropriate secondary anti-IgG antibody coupled to horseradish peroxidase were used.

    Article Title: 14-3-3 Is a Regulator of the Cardiac Voltage-Gated Sodium Channel Nav1.5
    Article Snippet: .. Co-immunoprecipitation, pulldown and Western blotting The African green monkey kidney derived COS-7 cells (obtained from ATCC), were transiently co-transfected with pCI Nav1.5-GFP and pCI 14-3-3η expression vectors using BGTC-DOPE (7). .. For each experimental condition, confluent cells from two 30 mm dishes were harvested after 2 days and incubated in 500 μL ice-cold lysis buffer (in mmol/L: 150 NaCl, 1% IGEPAL, 50 tris-HCl, pH 8.0; all from Sigma) with 1 minitab of protease inhibitors (Roche) and 1 mmol/L PMSF (phenylmethylsulfonyl fluoride, Sigma) for 1 h at 4°C.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Mutation in ST6GALNAC5 identified in family with coronary artery disease
    Article Snippet: .. The vectors were transfected into African green monkey kidney derived COS-7 cells (ATCC, Rockville, MD, USA) and ST6GALNAC5 expression in transfected cells was shown by RT-PCR and by Western blotting. .. In the Western blot experiments, monoclonal mouse M2-anti-FLAG (Sigma-Aldrich, Munich, Germany), anti-human sialyltransferase 7e rabbit (abcam, Cambridge, MA, USA), or anti-human lamin B goat (Santa Cruz Biotechnology, Santa Cruz, CA, USA) primary antibody and appropriate secondary anti-IgG antibody coupled to horseradish peroxidase were used.

    Western Blot:

    Article Title: Mutation in ST6GALNAC5 identified in family with coronary artery disease
    Article Snippet: .. The vectors were transfected into African green monkey kidney derived COS-7 cells (ATCC, Rockville, MD, USA) and ST6GALNAC5 expression in transfected cells was shown by RT-PCR and by Western blotting. .. In the Western blot experiments, monoclonal mouse M2-anti-FLAG (Sigma-Aldrich, Munich, Germany), anti-human sialyltransferase 7e rabbit (abcam, Cambridge, MA, USA), or anti-human lamin B goat (Santa Cruz Biotechnology, Santa Cruz, CA, USA) primary antibody and appropriate secondary anti-IgG antibody coupled to horseradish peroxidase were used.

    Article Title: 14-3-3 Is a Regulator of the Cardiac Voltage-Gated Sodium Channel Nav1.5
    Article Snippet: .. Co-immunoprecipitation, pulldown and Western blotting The African green monkey kidney derived COS-7 cells (obtained from ATCC), were transiently co-transfected with pCI Nav1.5-GFP and pCI 14-3-3η expression vectors using BGTC-DOPE (7). .. For each experimental condition, confluent cells from two 30 mm dishes were harvested after 2 days and incubated in 500 μL ice-cold lysis buffer (in mmol/L: 150 NaCl, 1% IGEPAL, 50 tris-HCl, pH 8.0; all from Sigma) with 1 minitab of protease inhibitors (Roche) and 1 mmol/L PMSF (phenylmethylsulfonyl fluoride, Sigma) for 1 h at 4°C.



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    (a) Schematic showing the experimental plan for CgA/PA interaction monitoring in living cells by the FLIM-FRET technique. Following the overexpression of CgA- or CgAΔPABD-mKate2 <t>in</t> <t>COS-7</t> cells, their secretion was induced during 5 min using a BaCl 2 solution. Then cells were washed and incubated 5 min with PA-ATTO647N. (b) Principle of FLIM-FRET technique to track the lifetime of the fluorescence donor (mKate2) without or with CgA/PA-ATTO647N interaction. The more CgA-mKate2 interacts with PA, the more the lifetime of mKate2 decreases due to its energy transfer to the acceptor fluorophore (ATTO647N). (c) Confocal observations of COS-7 cells overexpressing CgA-mKate2 or CgAΔPABD-mKate2 (green) after secretion stimulation and incubation with the PA-ATTO647N probe (magenta). Scale bars: 10 µm. The regions delimited by a white square on left images are enlarged to show the distribution of each fluorescent molecule. Scale bars: 2 µm. (d) Confocal observations of COS-7 cells overexpressing CgA-mKate2 or CgAΔPABD-mKate2, after cell incubation with PA-ATTO647N, showing the intensity of mKate2 fluorescence (grey), and mKate2 lifetime between 3 and 1.8 ns (rainbow color bar). The regions delimited by a white square are enlarged to show mKate2 lifetime at the plasma membrane. Scale bars: 5 µm. (e) Representative phasor plots showing the CgA-mKate2 lifetime at the level of the whole image and at the plasma membrane zone. Green line corresponds to 2.4 ns and yellow line to 2 ns, used as visual landmarks. (f) Representative phasor plot showing the CgAΔPABD-mKate2 lifetime at the level of the whole image. Green line corresponds to 2.4 ns and yellow line to 2 ns, used as visual landmarks. (g) Quantification of CgA-mKate2 or CgAΔPABD-mKate2 lifetime at the level of the plasma membrane (PM) or secretory granule (SG) without (-) or with (+) PA-ATTO647N. nd: not detected. Kruskall-Wallis test * p<0,05, each point representing the mean mKate2 lifetime in a ROI at the PM or SG level of 2 to 3 cells.
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    99
    ATCC african green monkey kidney derived cell line cos
    (a) Schematic showing the experimental plan for CgA/PA interaction monitoring in living cells by the FLIM-FRET technique. Following the overexpression of CgA- or CgAΔPABD-mKate2 <t>in</t> <t>COS-7</t> cells, their secretion was induced during 5 min using a BaCl 2 solution. Then cells were washed and incubated 5 min with PA-ATTO647N. (b) Principle of FLIM-FRET technique to track the lifetime of the fluorescence donor (mKate2) without or with CgA/PA-ATTO647N interaction. The more CgA-mKate2 interacts with PA, the more the lifetime of mKate2 decreases due to its energy transfer to the acceptor fluorophore (ATTO647N). (c) Confocal observations of COS-7 cells overexpressing CgA-mKate2 or CgAΔPABD-mKate2 (green) after secretion stimulation and incubation with the PA-ATTO647N probe (magenta). Scale bars: 10 µm. The regions delimited by a white square on left images are enlarged to show the distribution of each fluorescent molecule. Scale bars: 2 µm. (d) Confocal observations of COS-7 cells overexpressing CgA-mKate2 or CgAΔPABD-mKate2, after cell incubation with PA-ATTO647N, showing the intensity of mKate2 fluorescence (grey), and mKate2 lifetime between 3 and 1.8 ns (rainbow color bar). The regions delimited by a white square are enlarged to show mKate2 lifetime at the plasma membrane. Scale bars: 5 µm. (e) Representative phasor plots showing the CgA-mKate2 lifetime at the level of the whole image and at the plasma membrane zone. Green line corresponds to 2.4 ns and yellow line to 2 ns, used as visual landmarks. (f) Representative phasor plot showing the CgAΔPABD-mKate2 lifetime at the level of the whole image. Green line corresponds to 2.4 ns and yellow line to 2 ns, used as visual landmarks. (g) Quantification of CgA-mKate2 or CgAΔPABD-mKate2 lifetime at the level of the plasma membrane (PM) or secretory granule (SG) without (-) or with (+) PA-ATTO647N. nd: not detected. Kruskall-Wallis test * p<0,05, each point representing the mean mKate2 lifetime in a ROI at the PM or SG level of 2 to 3 cells.
    African Green Monkey Kidney Derived Cell Line Cos, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/african+green+monkey+kidney+derived+cos+7+cells/COS-7/pmc06484144-469-1-12
    Average 99 stars, based on 1 article reviews
    african green monkey kidney derived cell line cos - by Bioz Stars, 2026-11
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    african green monkey kidney derived cos 7 cells  (ATCC)
    99
    ATCC african green monkey kidney derived cos 7 cells
    (a) Schematic showing the experimental plan for CgA/PA interaction monitoring in living cells by the FLIM-FRET technique. Following the overexpression of CgA- or CgAΔPABD-mKate2 <t>in</t> <t>COS-7</t> cells, their secretion was induced during 5 min using a BaCl 2 solution. Then cells were washed and incubated 5 min with PA-ATTO647N. (b) Principle of FLIM-FRET technique to track the lifetime of the fluorescence donor (mKate2) without or with CgA/PA-ATTO647N interaction. The more CgA-mKate2 interacts with PA, the more the lifetime of mKate2 decreases due to its energy transfer to the acceptor fluorophore (ATTO647N). (c) Confocal observations of COS-7 cells overexpressing CgA-mKate2 or CgAΔPABD-mKate2 (green) after secretion stimulation and incubation with the PA-ATTO647N probe (magenta). Scale bars: 10 µm. The regions delimited by a white square on left images are enlarged to show the distribution of each fluorescent molecule. Scale bars: 2 µm. (d) Confocal observations of COS-7 cells overexpressing CgA-mKate2 or CgAΔPABD-mKate2, after cell incubation with PA-ATTO647N, showing the intensity of mKate2 fluorescence (grey), and mKate2 lifetime between 3 and 1.8 ns (rainbow color bar). The regions delimited by a white square are enlarged to show mKate2 lifetime at the plasma membrane. Scale bars: 5 µm. (e) Representative phasor plots showing the CgA-mKate2 lifetime at the level of the whole image and at the plasma membrane zone. Green line corresponds to 2.4 ns and yellow line to 2 ns, used as visual landmarks. (f) Representative phasor plot showing the CgAΔPABD-mKate2 lifetime at the level of the whole image. Green line corresponds to 2.4 ns and yellow line to 2 ns, used as visual landmarks. (g) Quantification of CgA-mKate2 or CgAΔPABD-mKate2 lifetime at the level of the plasma membrane (PM) or secretory granule (SG) without (-) or with (+) PA-ATTO647N. nd: not detected. Kruskall-Wallis test * p<0,05, each point representing the mean mKate2 lifetime in a ROI at the PM or SG level of 2 to 3 cells.
    African Green Monkey Kidney Derived Cos 7 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/african+green+monkey+kidney+derived+cos+7+cells/COS-7/pm29777707-43-10-16
    Average 99 stars, based on 1 article reviews
    african green monkey kidney derived cos 7 cells - by Bioz Stars, 2026-11
    99/100 stars
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    Image Search Results


    (a) Schematic showing the experimental plan for CgA/PA interaction monitoring in living cells by the FLIM-FRET technique. Following the overexpression of CgA- or CgAΔPABD-mKate2 in COS-7 cells, their secretion was induced during 5 min using a BaCl 2 solution. Then cells were washed and incubated 5 min with PA-ATTO647N. (b) Principle of FLIM-FRET technique to track the lifetime of the fluorescence donor (mKate2) without or with CgA/PA-ATTO647N interaction. The more CgA-mKate2 interacts with PA, the more the lifetime of mKate2 decreases due to its energy transfer to the acceptor fluorophore (ATTO647N). (c) Confocal observations of COS-7 cells overexpressing CgA-mKate2 or CgAΔPABD-mKate2 (green) after secretion stimulation and incubation with the PA-ATTO647N probe (magenta). Scale bars: 10 µm. The regions delimited by a white square on left images are enlarged to show the distribution of each fluorescent molecule. Scale bars: 2 µm. (d) Confocal observations of COS-7 cells overexpressing CgA-mKate2 or CgAΔPABD-mKate2, after cell incubation with PA-ATTO647N, showing the intensity of mKate2 fluorescence (grey), and mKate2 lifetime between 3 and 1.8 ns (rainbow color bar). The regions delimited by a white square are enlarged to show mKate2 lifetime at the plasma membrane. Scale bars: 5 µm. (e) Representative phasor plots showing the CgA-mKate2 lifetime at the level of the whole image and at the plasma membrane zone. Green line corresponds to 2.4 ns and yellow line to 2 ns, used as visual landmarks. (f) Representative phasor plot showing the CgAΔPABD-mKate2 lifetime at the level of the whole image. Green line corresponds to 2.4 ns and yellow line to 2 ns, used as visual landmarks. (g) Quantification of CgA-mKate2 or CgAΔPABD-mKate2 lifetime at the level of the plasma membrane (PM) or secretory granule (SG) without (-) or with (+) PA-ATTO647N. nd: not detected. Kruskall-Wallis test * p<0,05, each point representing the mean mKate2 lifetime in a ROI at the PM or SG level of 2 to 3 cells.

    Journal: bioRxiv

    Article Title: Chromogranin A regulates the dynamics of neurosecretion through its interaction with phosphatidic acid

    doi: 10.64898/2026.01.29.699889

    Figure Lengend Snippet: (a) Schematic showing the experimental plan for CgA/PA interaction monitoring in living cells by the FLIM-FRET technique. Following the overexpression of CgA- or CgAΔPABD-mKate2 in COS-7 cells, their secretion was induced during 5 min using a BaCl 2 solution. Then cells were washed and incubated 5 min with PA-ATTO647N. (b) Principle of FLIM-FRET technique to track the lifetime of the fluorescence donor (mKate2) without or with CgA/PA-ATTO647N interaction. The more CgA-mKate2 interacts with PA, the more the lifetime of mKate2 decreases due to its energy transfer to the acceptor fluorophore (ATTO647N). (c) Confocal observations of COS-7 cells overexpressing CgA-mKate2 or CgAΔPABD-mKate2 (green) after secretion stimulation and incubation with the PA-ATTO647N probe (magenta). Scale bars: 10 µm. The regions delimited by a white square on left images are enlarged to show the distribution of each fluorescent molecule. Scale bars: 2 µm. (d) Confocal observations of COS-7 cells overexpressing CgA-mKate2 or CgAΔPABD-mKate2, after cell incubation with PA-ATTO647N, showing the intensity of mKate2 fluorescence (grey), and mKate2 lifetime between 3 and 1.8 ns (rainbow color bar). The regions delimited by a white square are enlarged to show mKate2 lifetime at the plasma membrane. Scale bars: 5 µm. (e) Representative phasor plots showing the CgA-mKate2 lifetime at the level of the whole image and at the plasma membrane zone. Green line corresponds to 2.4 ns and yellow line to 2 ns, used as visual landmarks. (f) Representative phasor plot showing the CgAΔPABD-mKate2 lifetime at the level of the whole image. Green line corresponds to 2.4 ns and yellow line to 2 ns, used as visual landmarks. (g) Quantification of CgA-mKate2 or CgAΔPABD-mKate2 lifetime at the level of the plasma membrane (PM) or secretory granule (SG) without (-) or with (+) PA-ATTO647N. nd: not detected. Kruskall-Wallis test * p<0,05, each point representing the mean mKate2 lifetime in a ROI at the PM or SG level of 2 to 3 cells.

    Article Snippet: African green monkey kidney fibroblast-derived COS-7 cells (American Type Culture Collection; CRL 1651) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, Thermo FisherScientific) supplemented with 5% fetal bovine serum (FBS, Sigma-Aldrich), 100 U/mL penicillin, 100 μg/mL streptomycin (Gibco, Thermo FisherScientific).

    Techniques: Over Expression, Incubation, Fluorescence, Clinical Proteomics, Membrane

    (a) TIRF-M imaging of COS-7 cells overexpressing and secreting CgA- and CgAΔPABD-pHluorin after 2 mM BaCl 2 stimulation. SG leading to exocytic events on whole cells are surrounded and indicated by yellow arrows. Scale bars: 10 µm. Below are represented typical images of CgA- or CgAΔPABD-pHluorin exocytosis events. (b) Quantification of the number of exocytosis events in COS-7 cells overexpressing CgA-pHluorin and CgAΔPABD-pHluorin after their stimulation. (n=4 independent experiments; 8 cells in CgA condition and 7 cells in CgAΔPABD condition). Data are represented as mean ± SEM. Each point is one analyzed cell and each color is an independent experiment. **p < 0.01, Mann-Whitney test. (c) Typical images of CgA-EGFP or CgAΔPABD-EGFP exocytosis events. Scale bar: 500 nm. (d) Curves represent the normalized variation of the fluorescence intensity of single exocytic events during 10 s from CgA-or CgAΔPABD-EGFP overexpressing COS-7 cells after 2 mM BaCl 2 stimulation. The mean ± SEM of 75 exocytosis of 5 different CgA-EGFP overexpressing cells in 3 independent experiments and the mean of 65 exocytic events of 7 different CgAΔPABD-EGFP overexpressing cells in 3 independent experiments. Data have been normalized between 0 (before exocytosis) and 1 (maximum intensity). (e) Plot representing the mean ± SEM of area under curves from CgA-EGFP or CgAΔPABD-EGFP secretion kinetic. Each point represents one exocytosis event, and one color represents one analyzed cell. ****p < 0.0001, Mann-Whitney test.

    Journal: bioRxiv

    Article Title: Chromogranin A regulates the dynamics of neurosecretion through its interaction with phosphatidic acid

    doi: 10.64898/2026.01.29.699889

    Figure Lengend Snippet: (a) TIRF-M imaging of COS-7 cells overexpressing and secreting CgA- and CgAΔPABD-pHluorin after 2 mM BaCl 2 stimulation. SG leading to exocytic events on whole cells are surrounded and indicated by yellow arrows. Scale bars: 10 µm. Below are represented typical images of CgA- or CgAΔPABD-pHluorin exocytosis events. (b) Quantification of the number of exocytosis events in COS-7 cells overexpressing CgA-pHluorin and CgAΔPABD-pHluorin after their stimulation. (n=4 independent experiments; 8 cells in CgA condition and 7 cells in CgAΔPABD condition). Data are represented as mean ± SEM. Each point is one analyzed cell and each color is an independent experiment. **p < 0.01, Mann-Whitney test. (c) Typical images of CgA-EGFP or CgAΔPABD-EGFP exocytosis events. Scale bar: 500 nm. (d) Curves represent the normalized variation of the fluorescence intensity of single exocytic events during 10 s from CgA-or CgAΔPABD-EGFP overexpressing COS-7 cells after 2 mM BaCl 2 stimulation. The mean ± SEM of 75 exocytosis of 5 different CgA-EGFP overexpressing cells in 3 independent experiments and the mean of 65 exocytic events of 7 different CgAΔPABD-EGFP overexpressing cells in 3 independent experiments. Data have been normalized between 0 (before exocytosis) and 1 (maximum intensity). (e) Plot representing the mean ± SEM of area under curves from CgA-EGFP or CgAΔPABD-EGFP secretion kinetic. Each point represents one exocytosis event, and one color represents one analyzed cell. ****p < 0.0001, Mann-Whitney test.

    Article Snippet: African green monkey kidney fibroblast-derived COS-7 cells (American Type Culture Collection; CRL 1651) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, Thermo FisherScientific) supplemented with 5% fetal bovine serum (FBS, Sigma-Aldrich), 100 U/mL penicillin, 100 μg/mL streptomycin (Gibco, Thermo FisherScientific).

    Techniques: Imaging, MANN-WHITNEY, Fluorescence